ChloroquineV1, France, Analysis, bibRecord, 000442

A new and simple method for studying the binding and ingestion steps in the phagocytosis of yeasts

Identifieur interne : 000442 ( France/Analysis ); précédent : 000441; suivant : 000443

A new and simple method for studying the binding and ingestion steps in the phagocytosis of yeasts

Auteurs : J. Giaimis [France] ; Y. Lombard [France] ; M. Makaya-Kumba [France] ; P. Fonteneau [France] ; P. Poindron [France]

Source :

Journal of Immunological Methods [ 0022-1759 ] ; 1992.

RBID : ISTEX:10782CCF9948DE85622A8068D3A12F86A010BB0C

English descriptors

Teeft :
- Academic press, Acta pathol, Alternative pathway, Autoclaved, Autoclaved yeasts, Biol, Blood clearance, Cell biol, Chloroquine, Culture medium, Cytochalasin, Deep violet color, Escherichia coil, Escherichia coli, Extracellular yeasts, Fetal calf serum, Flow cytometry, Identical experiments, Ingestion, Internalization steps, Intracellular, Intracellular yeasts, Leukocyte biol, Light microscopy, Macrophage, Macrophage membrane, Malu, Malu cells, Malu cells phagocytosing autoclaved yeasts, Mannose, Mannose receptors, Microbial surface, Microorganism, Mononuclear phagocytes, Ofek, Optimal ratio, Phagocytosing cells, Phagocytosis, Phagocytosis test, Pink yeast, Pink yeasts, Receptor, Receptor recycling, Simple method, Staining properties, Surface lectins, Tannic, Tannic acid, Tannic acid solution, Tannic acid treatment, Total number, Total yeasts, Violet yeasts, Yeast.

Abstract

Abstract: Autoclaved yeasts are stained light pink by May-Grünwald Giemsa (MGG). If treated with tannic acid solution just before MGG staining, they display a deep violet color. It seemed possible that these properties could be used to discriminate between extra- and intracellular yeasts in a phagocytosis test, extracellular yeasts being violet and intracellular yeasts being pink. To validate this protocol, quantitative studies of phagocytosis by MALU cells (a murine macrophage cell line) were performed in the presence or absence of drugs known to interfere with phagocytosis.After treatment of cells with cytochalasin B, the mean number of pink yeasts per cell decreased in a dose-dependent manner, the mean number of violet yeasts increased in a dose-dependent manner, whereas the total number of cell-associated yeasts remained almost unchanged whatever the dose used. After treatment with α-mannans or chloroquine, the mean numbers of both violet and pink yeasts decreased in a dose-dependent manner.These results confirmed that (i) violet yeasts are extracellular, (ii) autoclaved yeasts recognize lectin receptors, and (iii) unstained (pink) yeasts are intracellular. We show that this simple method can be used for quantitative light microscopic analysis of both the attachment and internalization steps in the phagocytosis of yeasts.

Url:

https://api.istex.fr/ark:/67375/6H6-KH1BHKBW-B/fulltext.pdf

DOI: 10.1016/0022-1759(92)90191-U

Affiliations:

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ISTEX:10782CCF9948DE85622A8068D3A12F86A010BB0C

Le document en format XML

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A new and simple method for studying the binding and ingestion steps in the phagocytosis of yeasts

A new and simple method for studying the binding and ingestion steps in the phagocytosis of yeasts

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri